Shigella spp. – Identification

Laboratory Fee Schedule
Procedure #: MAQ0113A
CPT: N/A

Synonym(s):	Shigellosis
Requisition Form	G-22-(Food); G-23
Test Description	BAX® PCR Screen and conventional biochemical test and enrichment to isolate and identify Shigella species
Pre-Approval Needed	DSHS Regulatory Agency OR Emerging and Acute Infectious Disease Unit Agency
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Food
Sample/Specimen Type for Testing	Food/milk samples Environmental Swabs
Minimum Volume/Size Required	100 grams (¼ pound)
Storage/Preservation Prior to Shipping	Food Place food sample in sterile zip-lock or whirlpack bag or other sterile plastic container. Store on ice. Environmental swabs Environmental swabs must be collected on appropriate swabs used for environmental testing (e.g., 3M™ Quick Swabs or Spongesicle type swabs or equivalent).
Transport Medium	N/A
Specimen Labeling	Sample Identification must match submission form.
Shipping and Specimen Handling Requirements	Ship samples on Ice and/or Ice Packs. Samples must be shipped for overnight delivery. Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
Method	BAX® Real-Time PCR Screen FDA Bacteriological Analytical Manual (BAM)- Chapter 6
Turn-around Time	14 Days
Interferences/Limitations	The sample should represent the outer surface as well as the interior of the food.
Common Causes for Rejection	Insufficient specimen Not collected by a Registered Sanitarian Improperly stored. Failure to meet shipping guidelines.
Additional Information	N/A