Laboratory Fee Schedule
Procedure #: MCR0317A
CPT: 87040, 87070, 87077
| Synonym(s): |
Rocky Mountain Spotted Fever, Endemic Typhus, Flea-borne Typhus, Rickettsia rickettsii, Rickettsia typhi |
| Requisition Form |
G-2A |
| Test Description |
Standard IFA using a two-stage sandwich immunofluorescence technique for the detection of IgG antibodies to Rocky Mountain Spotted Fever and/or Typhus Fever antigens. |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
Serum |
| Minimum Volume/Size Required |
1.0 mL |
| Storage/Preservation Prior to Shipping |
- Store serum at 2°C to 8°C for up to 48 hours.
- For storage longer than 48 hours, store at -20°C or below until tested.
|
| Transport Medium |
N/A |
| Specimen Labeling |
- Two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable).
- Additional DSHS Laboratory specimen labeling guidelines available online here.
|
| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contain in accordance with federal shipping regulations for infectious agents.
- If specimens will arrive at the Lab within 48 hours of collection, refrigerate at 2°C–8°C and ship overnight on cold packs.
- If specimens will arrive at the Lab more than 48 hours after time of collection, freeze at -20°C or colder, and ship overnight on dry ice.
- Additional DSHS Laboratory specimen shipping guidelines online here.
|
| Method |
Indirect Immunofluorescence Assay |
| Turn-around Time |
5 working days. |
| Interferences/Limitations |
Grossly hemolyzed, lipemic, or contaminated specimens are not acceptable for testing. |
| Common Causes for Rejection |
- Insufficient specimen quantity.
- Unacceptable specimen type or source.
- Improper shipping conditions
- Expired media or collection container.
- Expired reagents/media/collection container.
- Discrepancies between G-2A submission form and specimen label.
- Missing/incomplete/illegible submission form or label.
|
| Additional Information |
- In order to confirm an infection was recent, a convalescent phase sample should be submitted.
- The most convincing evidence of recent rickettsial disease is a fourfold rise in antibody titer between an acute phase serum and a convalescent phase serum.
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