Laboratory Fee Schedule
Procedure Number: MAA0025A
| Synonym(s): |
Listeriosis, culture |
| Requisition Form |
G-2B |
| Test Description |
Isolation of Listeria monocytogenes from clinical specimen (with reflex to definitive identification and whole genome sequencing (WGS) |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human (stool culture for L. monocytogenes is not performed)
|
| Sample/Specimen Type for Testing |
- Blood: Collect in red top, tiger top tube, or blood culture bottle
- CSF and amniotic fluid: collect in screw-top sterile container
- Tissue: collect by biopsy or autopsy procedures and place in screw top sterile container
- Placenta: collect specimen in screw-top sterile container
|
| Minimum Volume/Size Required |
- Blood, CSF, amniotic fluid: 1ml
- Tissue, placenta: “pea-sized” or larger
|
| Storage/Preservation Prior to Shipping |
Store refrigerated at 2–8°C until shipment. |
| Transport Medium |
N/A |
| Specimen Labeling |
- At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
|
| Shipping and Specimen Handling Requirements |
- Ship refrigerated at 2–8°C on cold packs for overnight delivery to Laboratory.
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contained in accordance with federal shipping regulations.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Laboratory does not supply collection kits or shipping materials.
- Additional DSHS Laboratory specimen shipping guidelines online here
|
| Method |
Culture |
| Turn-around Time |
5–10 days |
| Interferences/Limitations |
Specimen should be taken from patient prior to antimicrobial therapy.
|
| Common Causes for Rejection |
- Incorrect specimen collection or submission methods
- Mismatch(es) between patient identifiers on G-2B submission form and specimen label.
- Insufficient specimen growth
- Incorrect storage conditions
- Expired reagents/media/collection container
- Discrepancies between G-2B submission form and specimen label
- Missing/incomplete/illegible submission form
|
| Additional Information |
N/A |