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Norovirus Detection, PCR

Laboratory Fee Schedule
Procedure:  MZZ0450A
CPT: 87798

Synonym(s):

Norwalk-like virus, stool

Requisition Form

G-2B

Test Description

PCR test designed to detect the presence of RNA from human noroviruses and the Norwalk-like virus genera

Pre-Approval Needed

N/A

Supplemental Information Required

N/A

Supplemental Form(s)

N/A  

Performed on Specimens from (sources)

Human

Sample/Specimen Type for Testing

Stool (raw or in Cary-Blair transport medium)

Minimum Volume/Size Required

200µL to 400µL

Storage/Preservation Prior to Shipping
  • Raw stool should be shipped between 2°C–8°C on frozen ice packs, or frozen on dry ice. 

  • Ship raw stool in a clean, dry, sealable container.

  • Stool in Cary-Blair medium may be shipped between 2°C–8°C or ambient. 

  • Do not freeze Cary-Blair medium.

Transport Medium

N/A

Specimen Labeling
  • At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).

  • Three unique patient identifiers are preferred. 

  • Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)

  • Additional DSHS Laboratory specimen labeling guidelines available online here.

Shipping and Specimen Handling Requirements
  • Raw stool: Ship refrigerated at 2°C–8°C on frozen cold packs or frozen on dry ice for overnight delivery

  • Cary-Blair stool specimens may be shipped cold on frozen cold packs, or at ambient temperatures.

  • Do not freeze Cary-Blair stool specimens.

  • Use multiple frozen cold packs when shipping cold.

  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.

  • Category B Biological Substance, UN3373 shipping requirements apply.

  • Handle as an infectious agent, triple pack and use universal precautions. 

  • Submitters are responsible for packaging and shipping specimens according to regulatory requirements. 

  • Additional DSHS Laboratory specimen shipping guidelines online here.

Method

Real-time PCR

Turn-around Time

48 business hours from receipt of specimen.

Interferences/Limitations

Heme from hemoglobin interferes with PCR in this assay. From hemoglobin interferes with PCR in this assay.

Common Causes for Rejection
  • Insufficient specimen volume

  • Mismatch(es) between patient identifiers on G-2B submission form and specimen label 

  • Bloody stool

  • Dried stool

  • Stored in transport media or preservatives.

  • Improper storage conditions.

  • Raw stool specimens received at ambient temperature.

  • Cary-Blair specimens received frozen. 

  • Failure to meet submission/shipping guidelines.

  • Missing/incomplete/illegible submission form.

Additional Information

PCR test may be paired with bacteriological culture.