Laboratory Fee Schedule
Procedure Number: MAA0054A, MAA0033A
| Synonym(s): |
Listeriosis, Listeria monocytogenes |
| Requisition Form |
G-2B |
| Test Description |
Identification confirmation of Listeria isolates with reflex to Whole Genome Sequencing (WGS) if Listeria monocytogenes |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
Pure Listeria isolates from blood, CSF, amniotic fluid, tissue (biopsy/autopsy), placenta |
| Minimum Volume/Size Required |
At least one viable colony of pure isolate per plate/slant) |
| Storage/Preservation Prior to Shipping |
Store refrigerated at 2–8°C until shipment. |
| Transport Medium |
Any medium that supports the growth of Listeria spp. (e.g., blood agar, Heart Infusion agar) |
| Specimen Labeling |
- At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
|
| Shipping and Specimen Handling Requirements |
- Ship refrigerated at 2–8°C on cold packs for overnight delivery.
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contain in accordance with federal shipping regulations.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Laboratory does not supply collection kits or shipping materials.
- Additional DSHS Laboratory specimen shipping guidelines online here
|
| Method |
MALDI-TOF (mass spectrometry), Culture |
| Turn-around Time |
5–10 days. |
| Interferences/Limitations |
- Specimen should be taken from patient prior to antimicrobial therapy.
- Organisms must be viable for culture studies.
- Improper storage or shipping temperatures limit organism recovery.
|
| Common Causes for Rejection |
- Incorrect specimen collection methods
- Mismatch(es) between patient identifiers on G-2B submission form and specimen label.
- Insufficient specimen growth
- Non-viable isolates
- Mixed culture/contamination
- Incorrect storage conditions
- Expired reagents/media/collection container
- Missing/incomplete/illegible submission form
|
| Additional Information |
- If L. monocytogenes is identified, the specimen will be reflexed to WGS.
- Listeriosis is a notifiable condition and L. monocytogenes isolates are REQUIRED to be submitted to DSHS Laboratory per Texas Administrative Code (TAC) chapter 97.
- Mark the “IDEAS” box for the payor source on the G-2B submission form.
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