Laboratory Fee Schedule
Procedure #: MBP0232A
CPT: 87536
| Synonym(s): |
HIV-1 RNA Quantification, HIV-1 NAAT, HIV-1 RNA |
| Requisition Form |
G-2A |
| Test Description |
Nucleic Acid Amplification Test (NAAT) to detect and quantify Human Immunodeficiency Virus type 1 (HIV-1) RNA in serum and plasma |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
- Serum collected in Serum Separator tubes (SST) or red top tubes
- Plasma collected in purple top tubes with EDTA
|
| Minimum Volume/Size Required |
1200µL of plasma or serum |
| Storage/Preservation Prior to Shipping |
- Serum
- Store at 2°C to 8°C for up to 5 days
- Store at -15°C to -35°C for up to 90 days
- Plasma
- Store at 2°C to 8°C for up to 5 days.
- Store at -20°C to -70°C for up to 90 days
|
| Transport Medium |
N/A |
| Specimen Labeling |
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
|
| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions.
- Triple contained in accordance with federal shipping regulations for infectious agents.
Additional forms & resources
|
| Method |
RNA Quantification by Real-Time Transcription Mediated Amplification |
| Turn-around Time |
3 working days |
| Interferences/Limitations |
- Reliable results are dependent on adequate specimen collection, transport, storage, and processing.
- This assay has been validated for use as a quantitative assay with plasma only.
- This assay has been validated for use as a qualitative assay with plasma and serum
- Though rare, mutations within the highly conserved regions of the viral genome covered by the primers and/or probes in this assay may result in underquantification of or failure to detect the virus.
|
| Common Causes for Rejection |
- Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired media or collection container
- Discrepancies between specimen label and submission form
- Incomplete or missing submission form
|
| Additional Information |
N/A |