Laboratory Fee Schedule
Procedure: MBL0217A
CPT: 86803
| Synonym(s): |
Anti-HCV, HCV Antibody, Hepatitis C Ab, HCV with Reflex |
| Requisition Form |
G-2A |
| Test Description |
Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of antibodies (IgG and IgM) to Hepatitis C Virus (HCV) in human serum. |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
Serum collected in SST or red top tubes |
| Minimum Volume/Size Required |
500 µL |
| Storage/Preservation Prior to Shipping |
- Store at 2°C to 8°C for up to 5 days.
- Store frozen at -20°C or colder for long term storage.
|
| Transport Medium |
N/A |
| Specimen Labeling |
- Two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number)
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
|
| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple contained in accordance with federal shipping regulations for infectious agents.
- If specimens will arrive at the Lab within 5 days of collection, refrigerate at 2°C –8°C and ship overnight on cold packs.
- If specimens will arrive at the Lab more than 5 days after time of collection, freeze at -20°C or below, and ship overnight on dry ice.
- Additional DSHS Laboratory specimen shipping guidelines online here.
|
| Method |
Enzyme Linked Immunosorbent Assay (ELISA) |
| Turn-around Time |
4 working days |
| Interferences/Limitations |
Clean, non-hemolyzed specimens are preferred. Precipitates in specimen should be removed by centrifugation. Do not use heat treated specimens. |
| Common Causes for Rejection |
- Insufficient specimen quantity.
- Unacceptable specimen type or source.
- Improper shipping conditions.
- Expired media or collection container.
- Expired reagents/media/collection container.
- Discrepancies between G-2A submission form and specimen label.
- Missing/incomplete/illegible submission form or label.
|
| Additional Information |
- The presence of anti-HCV does not constitute a diagnosis of HCV but may be indicative of recent and/or past infection.
- A nonreactive result does not exclude the possibility of exposure to hepatitis C virus. Levels of anti-HCV may be undetectable in early infection.
- HCV antibody reactive specimens will automatically reflex to HCV RNA nucleic acid amplification testing (NAAT).
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