PCR: Francisella tularensis

Laboratory Fee Schedule
Procedure #: BTA0028
CPT: 87798

Synonym(s):	Francisella tularensis, francisella Tularemia, Rabbit fever
Requisition Form	G-27A.
Test Description	Detection of Francisella tularensis DNA by Real-time PCR
Pre-Approval Needed	Must notify BioThreat Team prior to sample submission: (512) 689-5537.
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Human Environmental
Sample/Specimen Type for Testing	Pure culture isolate Blood, Serum Aspirate Bone Marrow Respiratory Secretions
Minimum Volume/Size Required	Culture isolates 1 plate or slant 1 ml for fluids Liquids 1 ml for Solids 2 grams
Storage/Preservation Prior to Shipping	Culture isolates ambient, Clinical samples 2-8°C (cold), keep tissue moist
Transport Medium	N/A
Specimen Labeling	Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements	Ship according to Dangerous Good Regulations, IATA, and/or CFR 49. Handled as infectious agent using universal precautions. Triple contained in accordance with federal shipping regulations for infectious agents. See also Emergency Preparedness resources online. Notify BioThreat Team prior to sample submission: (512) 689-5537
Method	Real-time PCR
Turn-around Time	Preliminary result 1 day after receipt, final result 21 days
Interferences/Limitations	Improper storage or shipping temperatures may limit organism recovery.
Common Causes for Rejection	Insufficient quantity Unacceptable specimen type or source Improper shipping conditions Expired media or collection container Discrepancies between specimen label and submission form Incomplete or missing submission form
Additional Information	May be paired with Definitive Identification: Francisella tularensis.

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