Brucella spp. PCR

Laboratory Fee Schedule
Procedure #: BTA0025
CPT: 87798


Synonym(s):	Brucella species (abortus, canis, melitensis, suis) PCR, Brucellosis PCR
Requisition Form	G-27A
Test Description	Detection of Brucella species DNA by PCR
Pre-Approval Needed	Must notify BioThreat Team prior to sample submission: (512) 689-5537.
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Non-Human (Bacterial) Human Environmental
Sample/Specimen Type for Testing	Culture isolates Whole blood Serum Environmental specimens
Minimum Volume/Size Required	Culture isolates: 1 agar slant or plate Clinical samples: 1 mL
Storage/Preservation Prior to Shipping	Culture isolates 2-8°C (cold) Environmental samples Letters, powders, liquids, plant material Ambient Milk, cheese, animal blood 2-8°C (cold) Animal tissues -20°C (frozen)
Transport Medium	N/A
Specimen Labeling	Human Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) Identifiers on specimen must exactly match submission form. Non-Human or Environmental Sample identification must match submission form.
Shipping and Specimen Handling Requirements	Ship according to Dangerous Good Regulations, IATA, and/or CFR 49. Handled as infectious agent using universal precautions. Triple contained in accordance with federal shipping regulations for infectious agents. See also Emergency Preparedness resources online. Notify BioThreat Team prior to sample submission: (512) 689-5537.
Method	PCR
Turn-around Time	Preliminary result 1 day after receipt, final result 1- 21 days
Interferences/Limitations	Improper storage or shipping temperatures may limit organism recovery.
Common Causes for Rejection	Insufficient quantity Unacceptable specimen type or source Improper shipping conditions Expired media or collection container Discrepancies between specimen label and submission form Incomplete or missing submission form
Additional Information	May be paired with Definitive ID: Brucella species