Laboratory Fee Schedule
Procedure #: BTA0006
CPT: 87075
| Synonym(s): |
Clostridium botulinum culture, Botulism |
| Requisition Form |
G-27A |
| Test Description |
Culture identification for Clostridium botulinum |
| Pre-Approval Needed |
- DSHS Epidemiology consultation with physician required
- Must notify BioThreat Team prior to sample submission: (512) 689-5537
|
| Supplemental Information Required |
Patient history |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
- Non-Human (Bacterial)
- Human
- Environmental
- Food (associated with a positive clinical sample)
|
| Sample/Specimen Type for Testing |
- Anaerobic isolate
- Stool
- Gastric
- Water enema
- Wound swab
- Tissue
- Food (associated with a positive clinical sample)
|
| Minimum Volume/Size Required |
Feces
- Infant 10 g or 5 mL enema
- Adult 50 g or 5 mL enema
Vomitus or gastric aspirate
Food
Wound swab
Tissue
|
| Storage/Preservation Prior to Shipping |
2-8°C (refrigerated). Wound, isolate or tissue ambient
|
| Transport Medium |
Isolates sent in anaerobic transport media |
| Specimen Labeling |
Human
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
Environmental or Food
- Sample identification must match submission form.
|
| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handled as infectious agent using universal precautions.
- Triple contained in accordance with federal shipping regulations for infectious agents.
- See also Emergency Preparedness resources online.
- Notify BioThreat Team prior to sample submission: (512) 689-5537
|
| Method |
Conventional biochemical methods |
| Turn-around Time |
7-14 days |
| Interferences/Limitations |
Improper storage or shipping temperatures may limit organism recovery. |
| Common Causes for Rejection |
- Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired media or collection container
- Discrepancies between specimen label and submission form
- Incomplete or missing submission form
|
| Additional Information |
May be paired with Toxin Assay (Botulinum) and/or PCR – Botulinum toxin genes. |