Microbiology Laboratory Tests: O-P

  

Microbiology
Paracoccidioidomycosis (Serological – Forwarded by TDSHS to CDC for testing.)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  3 weeks Reference Range:  Nonreactive
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Single Serum
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  Prior notification is requested (512) 458-7760. A detailed patient history is required. Serum is sent to the CDC.  CF titer parallels severity.  Cross-reactions do occur.  Precipitin bands denote past or present disease.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:  Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86671 Fees:


Microbiology
Paragonimiasis (Serological – Forwarded by TDSHS to CDC for testing.)
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Test Includes:

Reporting

Results Available: 3 weeks Contact #s:

Reference

Method:  
Turnaround Time:  3 weeks Reference Range:  Nonreactive
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type:
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Single Serum
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  Prior notification is requested (512) 458-7760. A detailed patient history is required. Serum is sent to the CDC.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:  Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86682 Fees:


Microbiology
Paragonimiasis (Microscopic – Direct Wet Smears Concentration)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  3 days Reference Range:
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Feces
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Fecal specimens must be sent in formalin. Adult flukes must be sent in ethyl alcohol.  Referred material accepted from hospital, private, and reference labs.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:  Ambient (Room) temperature   Shipping Requirements:

Billing

CPT Code: 87210 Fees:


Microbiology
Parainfluenza (Culture – Isolation)
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Test Includes: Cell culture

Reporting

Results Available: 5-14 days Contact #s: 512-458-7594

Reference

Method: Cell Culture  
Turnaround Time:  5-14 days Reference Range:  No virus isolated
Limitations: Interpretation: A result of “No virus isolated” does not necessarily mean absence of a viral agent.  The success of virus isolation depends a great deal on the submission of the proper specimen, collected at the right time, adequately maintained, and shipped with the least possible delay.

Specimen Requirements

Specimen Collection: Specimens should be collected at an appropriate anatomic site and at the proper time after infection because viruses are generally shed for only a short period of time.  Refer to Specimen Collection by Type table for additional instructions.  Sample Type: See preferred specimen.
Volume/Amount Required: Swabs in 2-4 mLs of viral transport media. Preferred Specimen:  Throat Swab; Nasopharyngeal Secretions
Collection/Preservation: Viral transport media. Storage Instructions: Arriving < 48 hours after collection, store and send at 2-8° C. Arriving > 48 hours after collection, store and send at -70° C (dry ice).
Causes for Rejection: Specimens submitted on a preservative such as formalin. Sample Container: Sterile container
Sample Test Kit: Availability: Monday - Friday
Diagnostic Information:  Specimens are inoculated onto a variety of cell culture monolayers.  If characteristic CPE or hemadsorption is observed, confirmation of identification will be performed.

Specimen Submission

Required Request Form: G-2V Specimen Handling:
Transport Temperature:  
Arriving <48 hours:  2-8°C
Arriving >48 hours:  send on dry ice  
Shipping Requirements: Ship specimens in compliance with governmental regulations.

Billing

CPT Code: 87252 Fees:


Microbiology
Parainfluenza (Culture – Typing)
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Test Includes: Immunofluorescence

Reporting

Results Available: 5-14 days Contact #s: 512-458-7594

Reference

Method: Immunofluorescence  
Turnaround Time:  5-14 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: If transporting on dry ice, send 1-2 mLs. Preferred Specimen:  Cell culture isolate with CPE.
Collection/Preservation: Storage Instructions: If shipment of isolate will be delayed, store at -70° C.
Causes for Rejection:   Sample Container: Sterile cryovial.
Sample Test Kit: Availability:  Monday - Friday
Diagnostic Information:   Typing of clinical isolates is based on immunofluorescence tests using monoclonal antibodies to parainfluenza 1, 2, 3, and 4.

Specimen Submission

Required Request Form: G-2V Specimen Handling:
Transport Temperature:  Frozen isolate: on dry ice Shipping Requirements: Ship specimens in compliance with governmental regulations.

Billing

CPT Code: 87253 Fees:

  

  
Microbiology
PinwormSee also Enterobiasis
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Test Includes: Microscopic Examination

Reporting

Results Available: 3 days

Contact #s: (512) 458-7560

Reference

Method: Microscopic examination

 

Turnaround Time:  3 days

Reference Range:  No pinworm eggs found

Limitations:

Interpretation:

Specimen Requirements

Specimen Collection: pinworm prep kits; taped slides

Sample Type: Pinworm prep kits, taped slides

Volume/Amount Required: 1 slide/kit per patient

Preferred Specimen: Pinworm prep kits, taped slides

Collection/Preservation: Using strip of clear cellulose tape sticky side outward on a microscope slide, press firmly against the perianal folds.  The tape is then spread back over the slide adhesive side down.  Ship in slide carrier.  If using pinworm prep kit, following instructions given in kit.

Storage Instructions: ambient temperature

Causes for Rejection: Frosted tape used;

Sample Container: slide carrier, pinworm prep kit.

Sample Test Kit:

Availability: Monday-Friday

Diagnostic Information:See Enterobiasis

 

Specimen Submission

Required Request Form: G-2B

Specimen Handling: Handle using universal percautions

Transport Temperature: ambient temperature

Shipping Requirements: Slide carriers, diagnostic specimen

Billing

CPT Code: 87172

Fees:

Microbiology
Plague Culture (Isolation)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  4-14 days Reference Range:  None isolated
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Lymph node aspirate; Blood; Sputum; Tissue; CSF  
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Telephone Laboratory (512-776-3781) prior to shipping specimen.  Cultures must be held for 14 days before reporting as negative. See Yersinia pestis. Yersinia pestis is one of the agents listed on the Bioterrorism agents list.   See Bioterrorism agents (Clinical -Isolation)

Specimen Submission

Required Request Form: G-27A  Specimen Handling:
Transport Temperature:  <24 hrs, 2-8°C Shipping Requirements:

Billing

CPT Code: 87040, 87045, 87070 Fees:


Microbiology
Plague Culture (Identification)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  4-7 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Pure culture, safely contained
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Telephone Laboratory (512-776-3781) prior to shipping specimen. See Yersinia pestis.  Yersinia pestis is one of the agents listed on the Bioterrorism agents list.  See Bioterrorism agents (Referred -identification).

Specimen Submission

Required Request Form:  G-27A  Specimen Handling:
Transport Temperature:  Ambient (Room) temperature Shipping Requirements:

Billing

CPT Code: 87077 Fees:


Microbiology
Plague (Serological - Hemagglutination)
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Reporting

Results Available: Contact #s:

Reference

Method: Hemagglutination  
Turnaround Time:  5-7 days Reference Range:  <1:32 (Nonreactive)  
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 2 mL Serum Preferred Specimen:  Single Serum
Collection/Preservation: Red top or tiger top tube Storage Instructions: Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability: Testing is done as requested
Diagnostic Information:  The passive hemagglutination test is used to detect antibodies to Yersinia pestis. Plague serology tests have been based on the detection of antibody to whole cells and to the Y.pestis-specific F1 antigen. If a person or animal has been exposed to Y. pestis, a serum specimen taken at the proper time after exposure/infection will very likely contain anti-F1 antibodies. Confirmation of a reactive result is done using the passive hemagglutination inhibition (PHI) test. Antibody to the antigen is adsorbed out before adding the sensitized sheep red blood cells to the specimen. Agglutination of true positives in the PHI buffer will be inhibited because the free F1 antigen in the F1 buffer will react with anti-F1 and thus remove antibody that can react with the F1 bound to the sheep red blood cells.  

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature: Cold (2-8º C) on ice packs if received within 48 hours from time of collection. Frozen (≤-20°) on dry ice if received more than 48 hours from time of collection. Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86793 Fees:


Test Includes: Serological testing performed at the CDC with prior approval only.

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  Determined by CDC reports Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:  Pure Culture
Volume/Amount Required: One specimen per isolate Preferred Specimen:  Pure culture, safely contained
Collection/Preservation: Storage Instructions: Ambient Temperature
Causes for Rejection: Nonviable, broken in transport Sample Container: Agar slant in screw cap tube
Sample Test Kit: Availability: Monday - Thursday
Diagnostic Information:  See Streptococcus pneumoniae (Culture - Typing) for serotyping of isolates. Serotyping is performed by CDC with prior permission and available only when suspected Vaccination failure or outbreak situation exists. Recurrent infections in a single individual must be approved by CDC before submission. For confirmation of organism as Streptococcus pneumoniae, see Aerobic Bacterial Culture (Isolation).

Specimen Submission

Required Request Form: G-2B Specimen Handling: Infectious agents
Transport Temperature:  Ambient (Room) temperature Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents.

Billing

CPT Code: 87070, 87077 Fees:


Microbiology
Pneumocystosis (Microscopic – Giemsa Stain)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  2 days Reference Range:
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Open lung biopsy; Bronchial lavage
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Open lung biopsy specimen of choice. Induced sputum is not acceptable (exceptions for AIDS patients) occasionally organisms can be found in bronchial lavage specimens.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:  Ambient (Room) temperature Shipping Requirements:

Billing

CPT Code: 87205 Fees:


Microbiology
Primary Amebic Meningoencephalitis Envirnmental (Culture - Isolation)
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Test Includes: Culture, microscopic examination, transformation study

Reporting

Results Available: 10 days Contact #s: (512) 458-7560

Reference

Method: Culture. microscopic examination, transformation study
Turnaround Time:  10 days Reference Range:  No parasites found
Limitations: Transformation study must be performed to confirm as Naegleria Interpretation: By report

Specimen Requirements

Specimen Collection: Water Sample Type: Water
Volume/Amount Required: 500 mL Preferred Specimen:  Water
Collection/Preservation: Collect water in a clean, dry leak-proof container Storage Instructions: ambient temperature
Causes for Rejection:  Environmental sample not linked with confirmed case. Sample Container:  Clean, dry, Leak-proof container
Sample Test Kit: Availability: Monday-Friday
Diagnostic Information:  Survey of environmental samples is provided only to health officials with prior arrangements. Culture and transformation studies can be performed.

Specimen Submission

Required Request Form: G-2B Specimen Handling: Handle according to guidelines for environmental samples.
Transport Temperature:  Ambient  temperature, do not refigerate   Shipping Requirements: Leak-proof container

Billing

CPT Code: 87081 Fees:


Microbiology
Primary Amebic Meningoencephalitis (Culture - Identification)
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Test Includes:

Reporting

Results Available: 1 day for direct exam; up to 10 days for culture. Contact #s: (512) 458-7560

Reference

Method:  
Turnaround Time:  1 day direct examination, up to 10 day Reference Range: No parasites found
Limitations: Interpretation:  By report

Specimen Requirements

Specimen Collection: spinal tap, biopsy Sample Type: CSF, brain biopsy
Volume/Amount Required: 1-2 mL spinal fluid, small piece of brain tissue. Preferred Specimen:  Spinal Fluid, brain biopsy  
Collection/Preservation: Collect by spinal tap, 1-2 mL CSF. By biopsy, collect small piece of brain tissue. Hold at ambient temperature. Storage Instructions: Ambient temperature, do not refrigerate or freeze.
Causes for Rejection: Insufficient specimen; name on tube/submission form do not match. Sample Container: Sterile, leak-proof container
Sample Test Kit: Availability:  Monday-Friday
With prior notification, Saturday.
Diagnostic Information:  Organisms can be cultured on nonnutrient agar plated with Eschericia coli. Direct examination of CSF and Trichrome stain can be performed.

Specimen Submission

Required Request Form:  G-2B Specimen Handling: Handling body fluids using universal precautions.
Transport Temperature:  Never refrigerate specimen.  Ambient temperature Shipping Requirements:  Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87081 Fees:


Microbiology
Primary Amebic Meningoencephalitis (Serological – Forwarded by TDSHS to CDC for testing.)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  3 weeks Reference Range:  Nonreactive
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Single Serum
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection:  Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  Prior notification is requested (512) 458-7760. A detailed patient history must be included. Serum specimens are sent to the CDC with prior arrangement only.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:  Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen).   Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86849 Fees:


Microbiology
Protothecosis (Culture - Isolation)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time: Reference Range:
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Isolate; Tissue
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Specimens are forwarded through this Laboratory to the CDC for fluorescence.

Specimen Submission

Required Request Form: G-2A Specimen Handling:
Transport Temperature: Shipping Requirements:

Billing

CPT Code: 87081 Fees:


Microbiology
Protothecosis (Microscopic – Direct Fluorescent Antibody)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time: Reference Range:
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Tissue
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Specimens are forwarded through this Laboratory to the CDC for fluorescence.

Specimen Submission

Required Request Form: G-2A Specimen Handling:
Transport Temperature: Shipping Requirements:

Billing

CPT Code: 87299 Fees:


Microbiology
Psittacosis (Serological – Forwarded by TDSHS to CDC for testing.)
Related Agents: Parrot Fever
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  3 weeks Reference Range:  Nonreactive
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Paired Sera
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  Birds must be sent to Texas A&M University, School of Veterinary Medicine.  Human serum specimens collected 14 days apart are forwarded to the CDC with prior arrangement.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:  Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code:  86631, 86632 Fees:
Last updated June 30, 2022