Microbiology Laboratory Tests: Hepatitis to Hookworm
Microbiology
Hepatitis A (Serological – Enzyme Immunoassay for HAVAb-total and HAV IgM)
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| Test Includes: Enzyme Immunoassay for Hepatitis A antibodies. | |
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Reporting |
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| Results Available: | Contact #s: |
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Reference |
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| Method: EIA | |
| Turnaround Time: 5-7 days | Reference Range: <1.00 (Nonreactive) |
| Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. | Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection. |
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Specimen Requirements |
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| Specimen Collection: Venipuncture | Sample Type: Serum |
| Volume/Amount Required: 2 mL Serum | Preferred Specimen: Single Serum |
| Collection/Preservation: Red top or tiger top tube | Storage Instructions: Separated serum may be held at 2-8°C. |
| Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing | Sample Container: Red top or tiger top tube. |
| Sample Test Kit: | Availability: Test run once per week. |
| Diagnostic Information: Tests are available only to Department’s epidemiologists to investigate outbreaks. Test is performed once per week. | |
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Specimen Submission |
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| Required Request Form: G-2A | Specimen Handling: Use Universal Precautions |
| Transport Temperature: Cold (2-8º C) on ice packs if received within 48 hours from time of collection. Frozen (≤-20°) on dry ice if received more than 48 hours from time of collection. | Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice. |
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Billing |
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| CPT Code: 86708 - Total, 86709 - IgM | Fees: |
Microbiology
Hepatitis B (Serological – Enzyme Immunoassay for HbsAg,and Anti-HBsAg, Total antibody or HBcAb-Total of IgM)
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| Test Includes: | |
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Reporting |
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| Results Available: | Contact #s: |
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Reference |
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| Method: EIA | |
| Turnaround Time: 1-2 days for HbsAg, 3-4 days for Anti-HbsAg and 5-7 days for HbcAb-Total | Reference Range: <1.00 (Nonreactive) |
| Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. | Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection. |
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Specimen Requirements |
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| Specimen Collection: Venipuncture | Sample Type: Serum |
| Volume/Amount Required: 2 mL Serum | Preferred Specimen: Single Serum |
| Collection/Preservation: Red top or tiger top tube | Storage Instructions: Separated serum may be held at 2-8°C. |
| Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing | Sample Container: Red top or tiger top tube. |
| Sample Test Kit: | Availability: Tests run daily. |
| Diagnostic Information: | |
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Specimen Submission |
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| Required Request Form: G-2A | Specimen Handling: Use Universal Precautions |
| Transport Temperature: Cold (2-8º C) on ice packs if received within 48 hours from time of collection. Frozen (≤-20°) on dry ice if received more than 48 hours from time of collection. | Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice. |
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Billing |
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| CPT Code: 86704 - Core, 86706 - sAb, 87340 - sAg | Fees: |
Microbiology
Hepatitis C (Serological – Enzyme Immunoassay)
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| Test Includes: | |
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Reporting |
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| Results Available: | Contact #s: |
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Reference |
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| Method: EIA | |
| Turnaround Time: 3-4 days | Reference Range: <0.620 |
| Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. | Interpretation: |
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Specimen Requirements |
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| Specimen Collection: Venipuncture | Sample Type: Serum |
| Volume/Amount Required: 5 mL Serum | Preferred Specimen: Single Serum |
| Collection/Preservation: Red top or tiger top tube | Storage Instructions: Separated serum may be held at 2-8°C. |
| Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing | Sample Container: Red top or tiger top tube. |
| Sample Test Kit: | Availability: test run 3 times weekly. |
| Diagnostic Information: Hepatitis C virus (HCV) causes 50-75% of cases described as non-A non-B hepatitis. The virus is spread primarily via blood and blood products. Known high-risk groups for HCV hepatitis include transfusion recipients, intravenous drug users, individuals with “street” tattoos and health care workers with frequent body fluid contact. Test is performed 3 times per week. | |
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Specimen Submission |
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| Required Request Form: G-2A | Specimen Handling: Use Universal Precautions |
| Transport Temperature: Separated serum or SST tubes at 2-8°C (refrigerated) or serum -20°C (frozen). | Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice. |
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Billing |
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| CPT Code: 86803, 86804 (RIBA) | Fees: |
Microbiology
Herpes Simplex (Culture - Isolate)
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| Test Includes: Cell culture | |
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Reporting |
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| Results Available: 1-12 days | Contact #s: 512-458-7594. |
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Reference |
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| Method: Cell culture | |
| Turnaround Time: 1-12 days | Reference Range: Herpes simples not isolated |
| Limitations: | Interpretation: A result of “Herpes simplex virus not isolated” does not necessarily mean absence of disease. The success of virus isolation depends a great deal on the submissions of the proper specimen, collected at the right time, adequately maintained, and shipped with the least possible delay. |
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Specimen Requirements |
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| Specimen Collection: Specimens should be collected at an appropriate anatomic site and at the proper time after infection because viruses are generally shed for only a short period of time. Refer to Specimen Collection by Type table for additional instructions. | Sample Type: See preferred specimen. |
| Volume/Amount Required: Swab in 2-4 mL of viral transport media, or tissue in enough viral transport media to prevent drying. | Preferred Specimen: Vesicular fluid/swab; Tissue. |
| Collection/Preservation: Viral transport media. | Storage Instructions: Arriving < 48 hours after collection, store and send at 2-8° C. Arriving > 48 hours after collection, store and send at -70° C (dry ice). |
| Causes for Rejection: Specimens submitted on a preservative such as formalin. | Sample Container: Sterile container. |
| Sample Test Kit: | Availability: Monday - Friday |
| Diagnostic Information: Isolation services are limited to designated public health programs. A variety of cell monolayers which support the growth of herpes simplex are inoculated and observed for 10 days. If characteristic CPE is observed, confirmation of identification will be performed. | |
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Specimen Submission |
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| Required Request Form: G-2V | Specimen Handling: |
| Transport Temperature: Arriving <48 hours: 2-8° C Arriving >48 hours: send on dry ice |
Shipping Requirements: Ship specimens in compliance with governmental regulations. |
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Billing |
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| CPT Code: 87252 | Fees: |
Microbiology
Herpes Simplex (Culture - Identification)
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| Test Includes: Immunofluorescence | |
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Reporting |
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| Results Available: 1-12 days | Contact #s: 512-458-7594. |
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Reference |
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| Method: Immunofluorescence | |
| Turnaround Time: 1-12 days | Reference Range: By report |
| Limitations: | Interpretation: |
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Specimen Requirements |
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| Specimen Collection: | Sample Type: |
| Volume/Amount Required: If transporting on dry ice, send 1-2 mL. | Preferred Specimen: Cell culture isolate with CPE. |
| Collection/Preservation: | Storage Instructions: If shipment of isolate will be delayed, store at -70° C. |
| Causes for Rejection: | Sample Container: Sterile cryovial. |
| Sample Test Kit: | Availability: Monday - Friday |
| Diagnostic Information: Isolation services are limited to designated public health programs.Identification is based on immunofluorescence test using monoclonal antibodies. | |
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Specimen Submission |
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| Required Request Form: G-2V | Specimen Handling: |
| Transport Temperature: Frozen isolate: on dry ice | Shipping Requirements: Ship specimens in compliance with governmental regulations. |
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Billing |
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| CPT Code: 87273, 87274 | Fees: |
Microbiology
HIV-1, Screen (Serological – Enzyme Immunoassay)
Related Agents: Acquired Immunodeficiency Virus
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| Test Includes: Enzyme Assay (EIA) | |
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Reporting |
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| Results Available: 1 day | Contact #s: |
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Reference |
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| Method: EIA | |
| Turnaround Time: 1-2 days | Reference Range: Nonreactive |
| Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. | Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection. |
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Specimen Requirements |
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| Specimen Collection: Venipuncture | Sample Type: |
| Volume/Amount Required: 5 mL Serum, or filter paper with 3 circles completely filled. | Preferred Specimen: Serum, plasma; Oral Fluid; Filter Paper Blood Spot |
| Collection/Preservation: Red top or tiger top tube | Storage Instructions: Separated serum may be held at 2-8°C. |
| Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing | Sample Container: Red top or tiger top tube. |
| Sample Test Kit: | Availability: Test performed daily. |
| Diagnostic Information: The EIA procedure for the detection of antibodies to HIV is a screening procedure. All positive or borderline results require validation by the Western Blot Confirmatory procedure. The western blot will automatically be performed and charged on all specimens with positive or borderline results. Specimens with Nonreactive EIA results will not be tested by western blot unless specifically requested. | |
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Specimen Submission |
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| Required Request Form: G-2A, G-THSTEPS for THSteps Medicaid | Specimen Handling: Use Universal Precautions |
| Transport Temperature: Separated serum or SST tubes at 2-8°C (refrigerated) or serum -20°C (frozen). Dried blood spots ambient up to 7 days. | Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice. |
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Billing |
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| CPT Code: 86701 | Fees: |
Microbiology
Hookworm (Microscopic – Direct Wet Smears Concentration)
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| Test Includes: Microscopic examination. | |
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Reporting |
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| Results Available: 3 days | Contact #s: (512) 458-7560. |
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Reference |
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| Method: Microscopic examination | |
| Turnaround Time: 3 days | Reference Range: No parasites found. |
| Limitations: | Interpretation: No parasite found indicates that there were no visible parasites in the specimen submitted. |
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Specimen Requirements |
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| Specimen Collection: feces | Sample Type: Feces, unpreserved (less than 5 hours), formalin preserved. |
| Volume/Amount Required: 15 mL liquid stool, 15 g stool | Preferred Specimen: Formalin preserved feces. |
| Collection/Preservation: Collect liquid stool or stool and immediately place into formalin preservative. Hold at ambient temperature. | Storage Instructions: Ambient temperature |
| Causes for Rejection: Unpreserved stool > 5 hours. | Sample Container: Formalin transport for parasites. |
| Sample Test Kit: | Availability: Monday-Friday |
| Diagnostic Information: Demonstration of eggs in formalin preserved stool samples. If stool is unpreserved for over 24 hours, the eggs may continue to develop, and the larvae may hatch for identification. | |
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Specimen Submission |
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| Required Request Form: G-2A | Specimen Handling: Handle body fluids using universal precautions. Formalin is considered a poison. |
| Transport Temperature: | Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens. |
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Billing |
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| CPT Code:87177 | Fees: |