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Synonym(s):
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Passive Hemagglutination and Inhibition
Tests for Detecting Anti-Yersinia pestis F-1 Antigen Antibodies
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Requisition
Form
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G-2A
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Test
Description
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Agglutination tests are simple methods
used for the visible detection of antigen-antibody complexes.
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Pre-Approval
Needed
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N/A
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Supplemental
Information Required
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N/A
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Supplemental
Form(s)
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N/A
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Performed on
Specimens from (sources)
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Human
Animal |
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Sample/Specimen
Type for Testing
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-
Serum
- Plasma in lavender EDTA tubes
- Blood dried on Nobuto strips
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Minimum
Volume/Size Required
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200 µL or one Nobuto strip
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Storage/Preservation
Prior to Shipping
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Serum or Plasma - Stored between 2°C to 8°C for no longer than 48 hours
- Over 48 hours, must be frozen at -20°C or colder
Nobuto Strips
- Keep at ambient room temperature and dry.
- If not used within 3 months, store strips at -20°C.
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Transport
Medium
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N/A
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Specimen
Labeling
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- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
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Shipping and
Specimen Handling Requirements
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- Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49.
- Handle
as infectious agent using universal precautions.
- Triple
contain in accordance with federal shipping regulations for infectious agents
Additional forms & resources
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Method
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Agglutination tests for the detection of
antibody-antigen complexes
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Turn-around
Time
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3 working days (Human specimens)
1 month (Nobuto strips)
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Interferences/Limitations
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Non-specific agglutination of heterophile
antibodies. Gross hemolysis, lipemia
and bacterial contamination may interfere.
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| Common Causes for
Rejection
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-
Insufficient
quantity
- unacceptable specimen type or source
- improper shipping conditions
- expired media or collection container
- discrepancies between specimen label
and submission form
- incomplete or missing submission form.
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Additional
Information
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A convalescent serum specimen is
recommended and should be taken at four weeks or longer in order to look for
seroconversion.
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