Synonym(s):
|
Passive Hemagglutination and Inhibition
Tests for Detecting Anti-Yersinia pestis F-1 Antigen Antibodies
|
Requisition
Form
|
G-2A
|
Test
Description
|
Agglutination tests are simple methods
used for the visible detection of antigen-antibody complexes.
|
Pre-Approval
Needed
|
N/A
|
Supplemental
Information Required
|
N/A
|
Supplemental
Form(s)
|
N/A
|
Performed on
Specimens from (sources)
|
Human
Animal |
Sample/Specimen
Type for Testing
|
-
Serum
- Plasma in lavender EDTA tubes
- Blood dried on Nobuto strips
|
Minimum
Volume/Size Required
|
200 µL or one Nobuto strip
|
Storage/Preservation
Prior to Shipping
|
Serum or Plasma - Stored between 2°C to 8°C for no longer than 48 hours
- Over 48 hours, must be frozen at -20°C or colder
Nobuto Strips
- Keep at ambient room temperature and dry.
- If not used within 3 months, store strips at -20°C.
|
Transport
Medium
|
N/A
|
Specimen
Labeling
|
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
|
Shipping and
Specimen Handling Requirements
|
- Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49.
- Handle
as infectious agent using universal precautions.
- Triple
contain in accordance with federal shipping regulations for infectious agents
Additional forms & resources
|
Method
|
Agglutination tests for the detection of
antibody-antigen complexes
|
Turn-around
Time
|
3 working days (Human specimens) 1 month (Nobuto strips)
|
Interferences/Limitations
|
Non-specific agglutination of heterophile
antibodies. Gross hemolysis, lipemia
and bacterial contamination may interfere.
|
Common Causes for
Rejection
|
-
Insufficient
quantity
- unacceptable specimen type or source
- improper shipping conditions
- expired media or collection container
- discrepancies between specimen label
and submission form
- incomplete or missing submission form.
|
Additional
Information
|
A convalescent serum specimen is
recommended and should be taken at four weeks or longer in order to look for
seroconversion.
|