Vibrio

V. parahaemolyticus & V. vulnificus (Foods, oysters) – MPN

Laboratory Fee Schedule

Procedure:  MAQ0217A 

CPT: N/A

 

V. parahaemolyticus & V. vulnificus (Foods, oysters) – MPN
Synonym(s): Vibrio
Requisition Form Program provides their own form or  G-22-(Food); G-23
Test Description BAX® PCR Screen and an FDA method of enumeration using a Most Probable Number (MPN) format followed by biochemical testing for identification of V. parahaemolyticus and V. vulnificus in oysters and other food samples
Pre-Approval Needed Regulatory or IDCU agent
Supplemental Information Required N/A
Supplemental Form(s) N/A  
Performed on Specimens from (sources) Food
Sample/Specimen Type for Testing
  • Oysters (shucked, frozen shucked, or in shell)
  • 100 grams (¼ pound)
Minimum Volume/Size Required
  • 10 to 12 animals, or 
  • 1 to 2 packages
  • 100 grams (¼ pound)
Storage/Preservation Prior to Shipping
  • Using sterile forceps/instrument, transfer sample to sterile, water-tight, wide-mouthed container. 
  • If possible, store sample at commercial-stock temperature; otherwise, store on crushed ice.
  • Place food sample in sterile zip-lock or whirlpack bag or other sterile plastic container.
  • Store on ice.
Transport Medium N/A
Specimen Labeling Sample Identification must match submission form.
Shipping and Specimen Handling Requirements
  • Ship samples on Ice and/or Ice Packs.
  • Samples must be shipped for overnight delivery.
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
Method
  • BAX® Real-Time PCR Screen
  • FDA Bacteriological Analytical Manual (BAM)- Chapter 9
Turn-around Time 14 Days
Interferences/Limitations

N/A

Common Causes for Rejection
  • insufficient specimen
  • not collected by a Registered Sanitarian
  • improperly stored
  • failure to meet shipping guidelines
Additional Information N/A

Last updated November 18, 2021