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Synonym(s):
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Enteric
pathogen identification, isolation
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Requisition Form
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G-2B |
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Test Description
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Enteric
pathogen identification and isolation from clinical specimens
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Pre-Approval Needed
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N/A
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Human
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Sample/Specimen Type for Testing
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Stool/fecal
specimens
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Minimum Volume/Size Required
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1ml
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Storage/Preservation Prior to Shipping
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- Collect raw stool
- Keep cold (unless if for Vibrio store at room
temp) or freeze immediately at -70˚C
- Place stool in transport medium: Follow manufacturer’s instructions for
storage and shipping
- If delays are expected, transport media may be
refrigerated (unless if for Vibrio store at room temp) or frozen at -70˚C
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Transport Medium
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Cary Blair,
GN Broth, etc. (For specimens in which a culture independent diagnostic test
(CIDT) was positive, and isolation is not performed, please send the original
material that yielded the positive result.)
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Specimen Labeling
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- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form
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Shipping and Specimen Handling Requirements
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- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Ship specimens overnight Monday-Thursday to avoid weekend deliveries.
- Ship refrigerated specimens on cold pack.
- Ship frozen specimens on dry ice.
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Method
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Bacterial culture
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Turn-around Time
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3-21 days
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Interferences/Limitations
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- Material must be viable. Improper storage or shipping temperatures limit
organism recovery.
- In the event of an outbreak, the procedure may be modified to
screen for an additional set of organisms.
- Where a specific agent of an outbreak has been previously
identified and there is a backlog of specimens, the procedure may be modified
to screen only for a specified organism.
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| Common Causes for Rejection
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-
Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired
media or collection container
- Discrepancies between specimen label and
submission form
- Incomplete or missing submission form.
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Additional Information
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- In the interest of public health, specimens
will be accepted up to 30 days from the date of collection in an effort to
identify the causative agent. The rate
of recovery decreases over time.
- Specimens with positive CIDT results will be
confirmed for the organism for which positive results were obtained. Please include results with submission.
- Routine stool culture includes screening for
Salmonella sp., Shigella sp., Vibrio sp., Campylobacter sp., Yersinia sp.,
and shiga-toxin producing E. coli.
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