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Synonym(s):
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Genetic
fingerprinting
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Requisition Form
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G-2B
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Test Description
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Test
visualizes the relatedness of various bacterial species by genetic
fingerprinting.
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Pre-Approval Needed
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- Prior approval for organisms that are submitted for nosocomial
investigations.
- Prior
approval needed for organisms from an environmental source, food source, or
other non-human source.
Contact
state epidemiologist at 512-776-6352.
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Supplemental Information Required
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If
specimen is Staphylococcus or Enterococcus, submitter must
specify if specimen is methicillin/ oxacillin-resistant or
vancomycin-resistant.
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Supplemental Form(s)
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- Include
copy of susceptibilities for drug-resistant organisms.
- For
isolates identified through culture independent diagnostic testing (CIDT-
e.g. molecular methods), include a copy of assay results.
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Performed on Specimens from (sources)
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Pure
bacterial isolates from human
- Environmental
- Food
Raw human
specimens such as stool are submitted to the Clinical Bacteriology Team. For, raw food and environmental sources, contact state epidemiologist.
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Sample/Specimen Type for Testing
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- Pure bacterial isolates from human, environmental, or food sources.
- Raw human specimens such as stool (submit to Clinical Bacteriology Team)
- Raw food and environmental sources (contact State Epidemiologist)
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Minimum Volume/Size Required
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At
least one colony of live growth
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Storage/Preservation Prior to Shipping
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Must
be live bacterial growth
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Transport Medium
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Any
media that sustains growth
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Specimen Labeling
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-
Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
- Identifiers on specimen must exactly match submission form.
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Shipping and Specimen Handling Requirements
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Ship samples: - According to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Handling as infectious agent using universal precautions
- Triple-contained in accordance with federal shipping regulations for infectious agents
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Method
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PFGE
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Turn-around Time
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14
business days from receipt (isolate may be sent to CDC for further study,
which will affect turnaround time.)
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Interferences/Limitations
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PFGE
strain typing is intended to provide laboratory evidence that
epidemiologically related isolates are also genetically related. Strain
typing data do not substitute for epidemiological data. Both should be
analyzed to determine whether an outbreak has occurred.
- Genetic
fingerprint pattern designations for each submitter are considered unique to
each submitter. Patterns are not
intended for comparison of relatedness with any other submitter, unless a
specific request is made for a special study.
- Limited
by database size for comparative studies of organism DNA patterns.
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| Common Causes for Rejection
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- No growth of isolate upon subculture
- Mixed isolate (more than one organism
submitted)
- No name on isolate
- At least two patient specific identifiers not
included
- Name mismatch (G2B form and isolate names do
not match)
- Isolate broke in transit
- Failure to meet submission/shipping guidelines
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Additional Information
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May
be paired with
- Salmonella
spp. – Serotyping;
- Shigella
spp. – Serotyping; and/or
- Bacterial
culture.
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