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Synonym(s):
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Non-O157
Shiga-Toxin producing Escherichia coli
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Requisition Form
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G-22-(Food);
G-23; MSA-FORM 50-1
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Test Description
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Food
analysis method approved by USDA FSIS for the detection, isolation, and
identification of “The Big Six” non-O157 STEC strains in Meat Samples and
Environmental Swabs.
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Pre-Approval Needed
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Regulatory
or IDCU agent
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Sample/Specimen Type for Testing
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- Food samples (ground meat, beef trim, carcass swab) samples
- Environmental
swabs
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Minimum Volume/Size Required
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500
grams (1.1 pounds) of raw ground beef/Beef Trim.
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Storage/Preservation Prior to Shipping
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Food
- Place
food sample in sterile zip-lock or whirlpack bag or other sterile plastic
container.
- Specimen
should be maintained at <15.0°C.
Environmental swabs
- Environmental
swabs must be collected on appropriate swabs used for environmental testing
(e.g., 3M™ Quick Swabs or Spongesicle type swabs or equivalent).
- Environmental swabs must be tested within 48 hours of collection.
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Transport Medium
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N/A
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Specimen Labeling
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Sample
Identification must match submission form.
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Shipping and Specimen Handling Requirements
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- Ship samples on Ice and/or Ice Packs.
- Samples must be shipped for overnight delivery.
- Ship according to Dangerous Good Regulations,
IATA, and/or CFR 49.
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Method
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- BAX® Real-Time PCR Screen
- USDA
FSIS culture method- Chapter 5B
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Turn-around Time
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14
Days
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Interferences/Limitations
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- The
sample should represent the outer surface as well as the interior of the
food.
- This
test only detects serogroups O26, O45, O103, O111, O121, and O145 (a.k.a.
“The Big Six”).
- Environmental
swabs must be tested within 48 hours of collection.
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| Common Causes for Rejection
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-
insufficient specimen
- not collected by a Registered Sanitarian
- incorrect swab used for collection
- improperly stored
- failure to meet shipping guidelines
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Additional Information
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N/A
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