Laboratory Fee Schedule
Procedure #: MAA0053A, MAA0054A
CPT: 87077
| Synonym(s): |
Enteric pathogen, Fermentative Gram-negative rod, Salmonella, Shigella, Enterobacteriales |
| Requisition Form |
G-2B for non-reportable organisms
|
| Test Description |
Identification of Enteric Bacteria from pure isolates (including notifiable condition isolates that are REQUIRED to be submitted per Texas Administrative Code (TAC) chapter 97.) |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
Pure isolate
|
| Minimum Volume/Size Required |
At least one viable colony
|
| Storage/Preservation Prior to Shipping |
Store at ambient temperatures or refrigerated at 2–8°C until shipment.
|
| Transport Medium |
Any appropriate medium that supports growth of enteric bacteria
|
| Specimen Labeling |
- At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, Medical record number)
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
|
| Shipping and Specimen Handling Requirements |
- Ship refrigerated at 2–8°C on cold packs for overnight delivery.
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contain in accordance with federal shipping regulations.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Laboratory does not supply collection kits or shipping materials.
- Additional DSHS Laboratory specimen shipping guidelines online here
- Ship specimens overnight Monday-Thursday to avoid weekend deliveries.
|
| Method |
- MALDI-TOF (mass spectrometry, Bacterial culture)
- Reflex to PCR or whole genome sequencing (WGS) is dependent upon organism identified.
|
| Turn-around Time |
2-21 days |
| Interferences/Limitations |
- Specimen should be taken from patient prior to antimicrobial therapy.
- Organisms must be viable for culture studies
- Improper storage or shipping temperatures limit organism recovery.
|
| Common Causes for Rejection |
- Incorrect specimen collection methods
- Mismatch(es) between patient identifiers on G-2B submission form and specimen label
- Insufficient specimen growth
- Improper shipping/storage conditions
- Expired media or collection container
- Discrepancies between G-2B submission form and specimen label
- Missing/Incomplete/Illegible submission form
|
| Additional Information |
- Salmonellosis is a notifiable condition and Salmonella isolates are REQUIRED to be submitted to DSHS Laboratory per Texas Administrative Code (TAC) chapter 97.
- Shigellosis is a notifiable condition, however, Shigella spp. isolates are not required to be submitted to DSHS Laboratory. Shigella spp. submissions are welcome.
- PCR and serotyping by WGS will be performed on received Shigella isolates.
- Mark the “IDEAS” box for the payor source in the G-2B submission form for all submitted isolates that are in the “required/requested” section of the submission form.
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