E. coli serotyping– Culture 

Laboratory Fee Schedule

Procedure #: MAA0010A

CPT: 87147

E. coli serotyping– Culture
Synonym(s): Serotyping
Requisition Form G-2B  for reportable organisms
Test Description Serotyping of Shiga-toxin positive Escherichia coli from reference culture or isolates from clinical samples
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing

Pure isolate, shiga-toxin positive clinical sample

Minimum Volume/Size Required

At least one viable colony

Storage/Preservation Prior to Shipping


Transport Medium

Appropriate medium that supports growth

Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Ship specimens overnight Monday-Thursday, to avoid weekend deliveries.
Method Bacterial culture
Turn-around Time 5-21 days
  • Material must be viable.
  • Improper storage or shipping temperatures limit organism recovery. 

Common Causes for Rejection

  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form.
Additional Information
  • Serotyping is performed from Shiga-toxin positive specimens only. 
  • Serotyping is available for the 7 most common serotypes. 
  • Specimens that are non-reactive with these serotypes will be submitted to the CDC for further testing.

Last updated July 8, 2020