Laboratory Fee Schedule
Procedure: BTA0201
CPT: 83789
| Synonym(s): | Clostridium botulinum toxin, Botulism |
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| Requisition Form | G-27A |
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| Test Description | Detection of Clostridium botulinum toxin |
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| Pre-Approval Needed | - DSHS Epidemiology consultation with physician required
- Must notify BioThreat Team prior to sample submission: (512) 689-5537
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| Supplemental Information Required | Patient history |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | - Human
- Environmental
- Food (associated with a positive clinical sample)
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| Sample/Specimen Type for Testing | - Anaerobic isolate
- Stool
- Gastric
- Water enema
- Serum (no serum on infants)
- Wound swab
- Tissue
- Food (associated with a positive clinical sample)
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| Minimum Volume/Size Required | Feces - Infant 10 g or 5 mL enema
- Adult 50 g or 5 ml enema
Vomitus or gastric aspirate Serum (no serum on infants) Food Wound swab Tissue |
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| Storage/Preservation Prior to Shipping | Wound swab, anaerobic culture or tissue - Maintain at ambient temperature
Serum - Maintain at 2° to 8°C (refrigerated) unless >48 hours, ship at -20°C (frozen)
All others - Maintain at 2° to 8°C (refrigerated)
Food - At existing storage temperature, collected by sanitarian only
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| Transport Medium | Isolates sent in anaerobic transport media |
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| Specimen Labeling | Human - Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
Environmental or Food - Sample identification must match submission form.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handled as infectious agent using universal precautions.
- Triple contained in accordance with federal shipping regulations for infectious agents.
- See also Emergency Preparedness resources online.
- Notify BioThreat Team prior to sample submission: (512) 689-5537
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| Method | Mass spectrometry |
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| Turn-around Time | 2-14 days |
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| Interferences/Limitations | Improper storage or shipping temperatures may affect toxin detection. |
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| Common Causes for Rejection | - Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired media or collection container
- Discrepancies between specimen label and submission form
- Incomplete or missing submission form
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| Additional Information | May be paired with Culture: Botulinum (human) and/or Real-Time PCR – Botulinum toxin genes |
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