|
Synonym(s):
|
N/A
|
|
Requisition Form
|
G-MYCO
|
|
Test Description
|
Test
for the identification of aerobic Actinomycete isolates, not Mycobacterium, referred
to DSHS Laboratory
|
|
Pre-Approval Needed
|
N/A
|
|
Supplemental Information Required
|
N/A
|
|
Supplemental Form(s)
|
N/A
|
|
Performed on Specimens from (sources)
|
Pure
isolate from human sources
|
|
Sample/Specimen Type for Testing
|
Aerobic
Actinomycete pure isolate in liquid or on solid culture medium
|
|
Minimum Volume/Size Required
|
- Liquid
culture - 2.0 mL
- Solid
culture - Single mature colony
|
|
Storage/Preservation Prior to Shipping
|
Ambient
|
|
Transport Medium
|
N/A
|
|
Specimen Labeling
|
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
|
|
Shipping and Specimen Handling Requirements
|
- Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49
- Category
B shipping applies for unidentified isolates
|
|
Method
|
- High performance liquid chromatography (HPLC)
|
|
Turn-around Time
|
HPLC
is one test in a battery of tests that take 3 to 4 weeks for full
identification.
|
|
Interferences/Limitations
|
- HPLC may only detect the predominant organism
in a mixed culture.
- HPLC cannot definitively identify an aerobic
Actinomycete.
- Additional testing methods are needed provide
definitive identification.
|
| Common Causes for Rejection
|
- Cultures
infested with mites
- Cultures
broken in transit
|
|
Additional Information
|
- Within one week of isolate receipt, and based on the HPLC analysis, the absence or presence of cell wall mycolic acids can be determined and/or a presumptive identification made based on mycolic acid pattern similarity to a given genus.
|