Synonym(s):
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Accuprobe, DNA probe
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Requisition Form
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G-MYCO
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Test Description
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Identification
Mycobacterium tuberculosis complex, Mycobacterium gordonae, Mycobacterium
avium complex, Mycobacterium kansasii by DNA probe
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Pre-Approval Needed
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No
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Pure
isolate from human sources
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Sample/Specimen Type for Testing
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Pure
isolate in liquid or on solid culture medium |
Minimum Volume/Size Required
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• Liquid culture 2.0 mL • Solid
culture - Visible growth
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Storage/Preservation Prior to Shipping
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Ambient
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Transport Medium
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N/A
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Specimen Labeling
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• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) • Identifiers on specimen must exactly match submission form.
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Shipping and Specimen Handling Requirements
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• Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49 • Category
B shipping applies for unidentified isolates
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Method
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Accuprobe culture
confirmation test; hybridization of ribosomal RNA to probe detected by
chemiluminesence
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Turn-around Time
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10 to 14 days for final report when included as
part of a referred isolate identification
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Interferences/Limitations
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• Positive
DNA Probe test does not mean the isolate is still viable.
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Common Causes for Rejection
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• Cultures
broken in transit • Culture
completely overgrown by mold
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Additional Information
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• Drug susceptibility tesing on M. avium
complex and M. gordonae not available at TDSHS laboratory. Isolates may be forwarded by TDSHS
laboratory to reference laboratory for susceptibility testing. A handling fee may be charged. Testing at other reference laboratories is
subject to fees charged by those laboratories.
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