|
Synonym(s): |
HPLC
Identification of clinical isolates |
|
Requisition Form
|
G-MYCO
|
|
Test Description
|
Mycolic
acid analysis identification of mycobacterial isolates cultured from clinical
specimens in DSHS Laboratory
|
|
Pre-Approval Needed
|
N/A
|
|
Supplemental Information Required
|
N/A
|
|
Supplemental Form(s)
|
N/A
|
|
Performed on Specimens from (sources)
|
Pure
isolate from human sources
|
|
Sample/Specimen Type for Testing
|
Mycobacterium
species recovered by DSHS laboratory in liquid or on solid culture medium
|
|
Minimum Volume/Size Required
|
• Liquid
culture- 2.0 mL • Solid
culture- Single mature colony
|
|
Storage/Preservation Prior to Shipping
|
N/A
|
|
Transport Medium
|
N/A
|
|
Specimen Labeling
|
• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) • Identifiers on specimen must exactly match submission form.
|
|
Shipping and Specimen Handling Requirements
|
N/A
|
|
Method
|
High
performance liquid chromatography
|
|
Turn-around Time
|
2
days
|
|
Interferences/Limitations
|
• HPLC may only detect the predominant organism
in a mixed culture. • Liquid cultures must be instrument positive in
order to analyze by HPLC. • Additional testing methods may need to be used
to provide definitive identification
|
| Common Causes for Rejection
|
N/A
|
|
Additional Information
|
• This test is used in-house to identify
Mycobacterium species cultured from submitted clinical specimens.
|