Synonym(s): |
HPLC
Identification of clinical isolates |
Requisition Form
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G-MYCO
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Test Description
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Mycolic
acid analysis identification of mycobacterial isolates cultured from clinical
specimens in DSHS Laboratory
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Pre-Approval Needed
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N/A
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Pure
isolate from human sources
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Sample/Specimen Type for Testing
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Mycobacterium
species recovered by DSHS laboratory in liquid or on solid culture medium
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Minimum Volume/Size Required
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• Liquid
culture- 2.0 mL • Solid
culture- Single mature colony
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Storage/Preservation Prior to Shipping
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N/A
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Transport Medium
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N/A
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Specimen Labeling
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• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) • Identifiers on specimen must exactly match submission form.
|
Shipping and Specimen Handling Requirements
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N/A
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Method
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High
performance liquid chromatography
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Turn-around Time
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2
days
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Interferences/Limitations
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• HPLC may only detect the predominant organism
in a mixed culture. • Liquid cultures must be instrument positive in
order to analyze by HPLC. • Additional testing methods may need to be used
to provide definitive identification
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Common Causes for Rejection
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N/A
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Additional Information
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• This test is used in-house to identify
Mycobacterium species cultured from submitted clinical specimens.
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