AFBBios

Acid Fast Bacilli Biochemicals, Basic 

Laboratory Fee Schedule 

              Procedure:MZZ0451A               

CPT: 87118


Acid Fast Bacilli Biochemicals, Basic
Synonym(s): Bios, Basic
Requisition Form G-MYCO
Test Description Identification of mycobacterial isolates referred to DSHS Laboratory by biochemical tests
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A  
Performed on Specimens from (sources) Pure isolate from human sources
Sample/Specimen Type for Testing Mycobacterium species pure isolate in liquid or on solid culture medium
Minimum Volume/Size Required
Liquid culture - 2.0 mL

Solid culture - Single mature colony

Storage/Preservation Prior to Shipping Ambient
Transport Medium N/A
Specimen Labeling

• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
• Identifiers on specimen must exactly match submission form. 

Shipping and Specimen Handling Requirements

•   Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49

•   Category B shipping applies for unidentified isolates

Method

•   Biochemical testing to detect the presence of absence of an enzyme

•   Physiological testing

Turn-around Time 14 days
Interferences/Limitations

•   Identification by biochemical tests is performed in conjunction with the morphological characteristics of the isolate and the HPLC pattern derived from cell wall analysis.

•   Biochemical testing can be affected by the age of the culture.

•   Identification by biochemical testing is limited and may not differentiate species without testing such as sequencing or mass spectrometry.

Common Causes for Rejection

•   Cultures overgrown by mold

•   Cultures broken in transit

Additional Information

Basic biochemical testing includes the most common biochemical panel used in the identification of Mycobacterium species.


Last updated February 9, 2022