Laboratory Fee Schedule
Procedure #: MZZ0399A
CPT: 87147,
| Synonym(s): | Serotyping, Grouping |
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| Requisition Form | G-2B. |
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| Test Description | Serotyping of Haemophilus influenzae. |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Pure isolates from sterile sites. |
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| Minimum Volume/Size Required | At least one viable colony. |
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| Storage/Preservation Prior to Shipping | After overnight incubation at 35°C –37°C w/ ~5% CO2, store slants at ambient temperature. |
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| Transport Medium | Appropriate solid medium that supports growth |
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| Specimen Labeling | - Two patient-specific identifiers required (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Ship overnight at ambient temperature.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contain in accordance with federal shipping regulations for infectious agents.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Additional DSHS Laboratory specimen shipping guidelines online here
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| Method | Slide agglutination serotyping |
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| Turn-around Time | 2–14 days |
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| Interferences/Limitations | - Isolates must be viable.
- Improper storage or shipping temperatures limit organism recovery.
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| Common Causes for Rejection | - Insufficient specimen quantity.
- Unacceptable specimen type or source.
- Improper shipping conditions.
- Expired media or collection container.
- Discrepancies between specimen label and G-2B specimen submission form.
- Incomplete or missing submission form(s).
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| Additional Information | - Only Isolates from invasive cases (normally sterile sites) from children <5 years old are required to be submitted per Texas Administrative code (TAC) chapter 97: §97.3(a)(4), §97.4(a)(6), and §97.5(a)(2)(C); see https://www.dshs.state.tx.us/idcu/investigation/conditions/.
- Isolates from normally sterile sites from patients >5 years old will also be accepted.
- Other sources may require epidemiology approval.
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