Haemophilus ducreyi Isolation and Identification

Laboratory Fee Schedule
Procedure Number: MAA0012A

Synonym(s):	Chancroid, genital ulcer, genital lesion, STD, STI, necrotic, bubo, lymph gland
Requisition Form	G-2B
Test Description	Isolation of Haemophilus ducreyi from genital lesions or abscess and identification via conventional biochemical methods.
Pre-Approval Needed	N/A
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Human
Sample/Specimen Type for Testing	Swab or scraping from base of lesion/ulcer or under scab; Abscess aspirate genital lesion, urethral swabs, aspirates from buboes
Minimum Volume/Size Required	N/A
Storage/Preservation Prior to Shipping	Store at 2–8°C until shipment. Ship to DSHS Laboratory within 24 hours of collection.
Transport Medium	Amies transport medium
Specimen Labeling	At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number). Three patient identifiers are preferred Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.) Additional DSHS Laboratory specimen labeling guidelines available online here.
Shipping and Specimen Handling Requirements	Ship refrigerated at 2–8°C on cold packs for overnight delivery to Laboratory. Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49. Category B Biological Substance, UN3373 shipping requirements apply. Handle as infectious agent using universal precautions. Triple-contained in accordance with federal shipping regulations. Submitters are responsible for packaging and shipping specimens according to regulatory requirements. Laboratory does not supply collection kits or shipping material Additional DSHS Laboratory specimen shipping guidelines online here
Method	Culture with reflex to Conventional biochemical identification
Turn-around Time	5–15 days
Interferences/Limitations	Specimen should be taken prior to antimicrobial therapy.
Common Causes for Rejection	Incorrect specimen collection or submission methods Mismatch(es) between patient identifiers on G-2B submission form and specimen label Incorrect storage conditions (Specimen must be shipped refrigerated on ice packs. Ambient temperature specimens are not acceptable Expired reagents/media/collection container Discrepancies between G-2B submission form and specimen label Missing/incomplete/illegible submission form
Additional Information	Intact bubo aspirates are rarely positive for H. ducreyi. Ruptured buboes are more likely to yield organisms.