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Escherichia coli Shiga Toxin Virulence Factor Detection- PCR

Laboratory Fee Schedule
Procedure #:  MMB0004A
CPT: 87150

Synonym(s):  E. coli PCR
Requisition Form G-2B
Test Description Real time polymerase chain reaction (PCR) for the detection of eight different virulence factors: stx1, stx2, uidA, eaeA, ipaH, ST, LT, E-hly
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources)
  • Human 
  • Food 
  • Environmental
Sample/Specimen Type for Testing Pure isolates are preferred
Minimum Volume/Size Required N/A
Storage/Preservation Prior to Shipping Plates or broth referred from Clinical Bacteriology Team
Transport Medium N/A
Specimen Labeling Human
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
Environmental or Food
  • Sample identification must match submission form.
Shipping and Specimen Handling Requirements Ship samples: 
  • according to Dangerous Good Regulations, IATA, and/or CFR 49
  • handling as infectious agent using universal precautions
  • triple-contained in accordance with federal shipping regulations for infectious agents
Method Real-time PCR
Turn-around Time 7 business days from receipt (if isolate is sent to CDC for further study, turnaround time may be longer)
Interferences/Limitations N/A
Common Causes for Rejection
  • Insufficient specimen growth
  • Mixed specimen
  • Failure to meet submission/shipping guidelines
Additional Information May be paired with bacteriological culture and/or PFGE