Laboratory Fee Schedule
Procedure #: MPS0008A
CPT: 86753
| Synonym(s): |
Trypanosoma cruzi IgG Antibody Immunoassay
Chagas Serologic Screen |
| Requisition Form |
G-2A |
| Test Description |
Qualitative detection of IgG antibodies against Trypanosoma cruzi, the causative agent of Chagas disease, using two separate indirect Enzyme-Linked Immunosorbent Assays (ELISA). |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
- Preferred Specimen:
- Serum collected in Serum Separator tubes (SST) or red top tubes
- Alternative Specimen:
- Plasma collected in purple top tubes with EDTA
|
| Minimum Volume/Size Required |
1 mL of serum or plasma |
| Storage/Preservation Prior to Shipping |
- Store at 2°C to 8°C for up to 7 days
- Store at -20°C or colder for up to 60 days
|
| Transport Medium |
N/A |
| Specimen Labeling |
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
|
| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions.
- Triple contained in accordance with federal shipping regulations for infectious agents. Additional forms & resources
|
| Method |
Indirect Enzyme-Linked Immunosorbent Assay (ELISA) |
| Turn-around Time |
7 working days |
| Interferences/Limitations |
- Hemolysis, hyperlipemia or other causes of turbidity may cause erroneous results.
- Heat-inactivated samples may yield false-positive results.
- A negative result does not exclude the possibility of exposure or infection by T. cruzi.
- Antibodies produced in response to other typanosomal infections may cross react with the T. cruzi antigen preparation used in one of the ELISA assays.
|
| Common Causes for Rejection |
- Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired media or collection container
- Discrepancies between specimen label and submission form
- Incomplete or missing submission form
|
| Additional Information |
- All Positive, Equivocal, and Inconclusive serum specimens will be forwarded to the CDC for confirmatory testing.
- Serum specimens preferred as the CDC does not accept plasma specimens for confirmatory testing.
|