Gram-negative rod identification - Culture
Laboratory Fee Schedule
Procedure #: MAA0032A
CPT: 87077
| Synonym(s): | GNR, non-enteric, non-fermentative rods, non-fermentative bacilli |
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| Requisition Form | G-2B |
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| Test Description | Identification of gram-negative, nonfermentative and non-enteric fermentative rods from reference culture. |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Pure isolate |
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| Minimum Volume/Size Required | At least one viable colony |
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| Storage/Preservation Prior to Shipping | Room temperature or refrigerated. |
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| Transport Medium | Appropriate medium that supports growth |
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| Specimen Labeling | - At least two patient identifiers are required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contain in accordance with federal shipping regulations.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Ship specimens overnight Monday-Thursday to avoid weekend deliveries.
- Laboratory does not supply collection kits or shipping materials.
- Additional DSHS Laboratory specimen labeling guidelines online here.
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| Method | Bacterial culture, MALDI-TOF (Mass Spectrometry) |
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| Turn-around Time | 3-21 days. |
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| Interferences/Limitations | Specimen must be a viable pure isolate for testing. |
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| Common Causes for Rejection | - Mismatch(es) between patient identifiers on G-2B submission form and specimen label.
- Non-viable isolates
- Mixed culture/contamination
- Improper /storage shipping conditions
- Expired media
- Missing/incomplete/illegible submission form
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| Additional Information | If Brucella or Francisella sp. is suspected, notify the Biothreat Team at 512-776-3781 and submit specimen with a completed G-27A submission form. |
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